Development of a realâ€time PCR system for the detection of the potential allergen fish in food
Fish is one of the most important triggers of food-allergic reactions. Regulation (EU) No 1169/2011 governs the labelling of allergenic ingredients includ- ing sh. A real-time PCR assay, using TaqMan® probes, was applied to detect sh in food. For generating primer and probes, the Hoxc13 gene was selected. Based on the alignment of available […]